Scientific Investigation

Understand experimental design and methodology

Scientific Investigation (ACT Science)

The Scientific Method

The scientific method is a systematic approach to understanding the natural world:

1. Observation → Notice something interesting
2. Question → Ask what causes it
3. Hypothesis → Propose testable explanation
4. Prediction → State what should happen if hypothesis is true
5. Experiment → Test the prediction
6. Analysis → Examine the data
7. Conclusion → Support or reject hypothesis

Types of Scientific Investigations

1. Controlled Experiments

Purpose: Test cause-and-effect relationships

Key features:

  • Independent variable: What you change
  • Dependent variable: What you measure
  • Control variables: What you keep constant
  • Control group: Baseline comparison

Example: Testing if fertilizer increases plant growth

  • Independent: Amount of fertilizer
  • Dependent: Plant height
  • Controls: Water, sunlight, soil type, plant species
  • Control group: Plants with no fertilizer

2. Observational Studies

Purpose: Observe without manipulating

Characteristics:

  • No variables are changed
  • Natural conditions
  • Look for patterns/correlations
  • Cannot prove causation

Example: Observing bird migration patterns

  • Record when birds arrive/depart
  • Note weather conditions
  • Track population changes

3. Comparative Investigations

Purpose: Compare two or more groups

Example: Comparing reaction rates at different temperatures

  • Test at 20°C, 30°C, 40°C, 50°C
  • Measure time for reaction completion
  • Look for relationship between temperature and rate

Variables in Investigations

Types of Variables

Independent Variable (IV):

  • What experimenter changes/controls
  • Goes on x-axis of graph
  • "Cause" in cause-effect

Dependent Variable (DV):

  • What's measured/observed
  • Responds to changes in IV
  • Goes on y-axis of graph
  • "Effect" in cause-effect

Control Variables:

  • Kept constant
  • Prevents confounding effects
  • Ensures fair test

Example Investigation:

"Students tested how light intensity affects photosynthesis rate. They placed plants under lights of different brightness and measured oxygen production."

  • IV: Light intensity (what they changed)
  • DV: Oxygen production (what they measured)
  • Controls: Plant type, temperature, CO₂ levels, water, time

ACT Tip: Identifying Variables

Question pattern: "What was the independent variable in Experiment 2?"

Strategy:

  1. Find Experiment 2 description
  2. Look for "Students changed..." or "tested at different..."
  3. That's your independent variable

Experimental Design Elements

Controls

Why control?

  • Isolates effect of independent variable
  • Eliminates alternative explanations
  • Makes results valid

Types:

  • Control group: Receives no treatment (baseline)
  • Control variables: Factors kept constant

Example: Testing new medicine:

  • Control group: Gets placebo
  • Experimental group: Gets medicine
  • Control variables: Age, dosage timing, diet

Sample Size

Larger samples = Better reliability

Why?

  • Reduces impact of outliers
  • Increases statistical significance
  • Makes patterns clearer

ACT questions might ask: "How could this experiment be improved?"

Good answer: "Test more subjects" or "Increase number of trials"

Precision and Accuracy

Precision: How close measurements are to each other

  • Consistent results
  • Small variation

Accuracy: How close measurements are to true value

  • Correct results
  • Measures what you intend

Improving precision:

  • Use better instruments
  • Take multiple measurements
  • Control environment

Replication

Why replicate?

  • Verify results aren't flukes
  • Increase confidence in findings
  • Identify errors

Two types:

  1. Repeated trials: Same researcher, same conditions
  2. Independent replication: Different researchers test same hypothesis

Data Collection and Analysis

Data Types

Quantitative: Numbers, measurements

  • Example: Temperature = 25°C, Mass = 150g

Qualitative: Descriptions, observations

  • Example: Color changed to blue, texture became rough

ACT favors quantitative data — easier to graph and analyze

Organizing Data

Tables:

  • Rows and columns
  • IV typically in left column
  • DV in right column(s)
  • Include units!

Graphs:

  • IV on x-axis
  • DV on y-axis
  • Title and axis labels essential
  • Show trends clearly

Identifying Patterns

Look for:

Positive correlation: Both variables increase together
Negative correlation: One increases as other decreases
No correlation: No clear relationship
Linear trend: Straight line relationship
Non-linear trend: Curved relationship

Drawing Conclusions

Valid Conclusions

Must be: ✓ Based on data
✓ Specific to conditions tested
✓ Acknowledge limitations
✓ Distinguish correlation from causation

Example of good conclusion: "In this experiment, increasing temperature from 20°C to 50°C decreased reaction time from 60 seconds to 15 seconds."

Poor conclusion: "Temperature always makes reactions faster." (Too general, ignores possible limits)

Limitations

Every investigation has limitations:

Sample size — Might be too small
Duration — Might be too short
Range — Might not test all conditions
Controls — Might miss confounding variables
Measurement error — Instruments have limits

ACT questions: "What is a limitation of this study?"

Strategy: Look for what wasn't controlled, what wasn't tested, or what could affect results

Common ACT Question Types

Type 1: Identify Variables

"In Experiment 1, the independent variable was:"

Strategy:

  • Find Experiment 1
  • Look for what was changed
  • That's the IV

Type 2: Improve Design

"Which change would improve this experiment?"

Common good answers:

  • Increase sample size
  • Add more trials
  • Include control group
  • Control additional variable
  • Use more precise measuring tool

Type 3: Support/Contradict Hypothesis

"Which result supports Hypothesis A?"

Strategy:

  • Understand what Hypothesis A predicts
  • Find data that matches that prediction
  • Check graphs/tables for confirming evidence

Type 4: Additional Investigation

"To further test this hypothesis, students should:"

Strategy:

  • Think about what wasn't tested yet
  • Look for logical next step
  • Must be testable and related

Common Investigation Flaws (ACT Favorites!)

Flaw 1: No control group

  • Can't tell if change was due to treatment

Flaw 2: Multiple variables changed

  • Can't tell which variable caused effect

Flaw 3: Too few trials

  • Results might not be reliable

Flaw 4: Bias in sample selection

  • Results might not be representative

Flaw 5: Improper measurement

  • Wrong tool or technique

ACT loves asking: "What is wrong with this experimental design?"

Experimental Ethics (Sometimes Tested!)

Key principles:

Informed consent: Participants know what they're agreeing to
Minimize harm: Don't cause unnecessary suffering
Confidentiality: Protect participant privacy
Honesty: Report results truthfully

Animal research: Minimize pain, use only when necessary

Quick Tips for ACT Science

Read the introduction carefully — tells you what's being investigated
Circle variables — mark IV, DV, and controls
Check sample size — larger is usually better
Look for controls — proper experiments need them
Note units — °C vs °F, cm vs m matters!
Follow the data — don't use outside knowledge
Check axes — make sure you know what graph shows
Process of elimination — often 2-3 choices are clearly wrong

Practice Approach

For investigation passages:

  1. Skim for structure — How many experiments? What's the overall question?
  2. Read intro — What's the research question?
  3. Identify variables — For each experiment, note IV, DV, controls
  4. Check graphs/tables — What do they show?
  5. Go to questions — Often easier than reading whole passage first
  6. Find relevant info — Locate specific experiment or data
  7. Answer from passage — Don't overthink!

Remember: ACT Science mostly tests whether you can read and understand scientific information, not whether you've memorized biology or chemistry facts. Focus on understanding the investigation design and what the data shows!

📚 Practice Problems

1Problem 1easy

Question:

A scientist wants to test whether fertilizer increases plant growth. Which is the best control group?

F) Plants with no fertilizer G) Plants with maximum fertilizer H) Plants in different temperatures J) Plants of different species K) No control group needed

💡 Show Solution

A control group receives no treatment to provide a baseline for comparison.

Experimental question: Does fertilizer increase plant growth?

Independent variable: Amount of fertilizer Dependent variable: Plant growth

Step 1: Define control group Control = baseline condition without the treatment being tested

Step 2: Evaluate options

F) "Plants with no fertilizer" • No treatment applied ✓ • Same as experimental except fertilizer ✓ • Perfect control! ✓ CORRECT!

G) "Plants with maximum fertilizer" • This is a treatment group, not control ✗

H) "Plants in different temperatures" • Introduces another variable ✗ • Not controlling for fertilizer effect ✗

J) "Plants of different species" • Introduces species variable ✗ • Can't isolate fertilizer effect ✗

K) "No control group needed" • Always need control for comparison! ✗

Answer: F) Plants with no fertilizer

Control group characteristics: • Identical to experimental group EXCEPT for variable being tested • Receives no treatment (or placebo) • Provides baseline for comparison • Helps prove treatment caused the effect

Experiment design: • Control group: Plants + water (no fertilizer) • Experimental group: Plants + water + fertilizer • Keep all else constant: same light, temp, water, soil

2Problem 2medium

Question:

An experiment tests three different concentrations of salt water (5%, 10%, 15%) on plant growth. What is the independent variable?

A) Plant growth B) Salt concentration C) Type of plant D) Amount of water E) Temperature

💡 Show Solution

Independent variable = what the experimenter CHANGES Dependent variable = what is MEASURED as a result

Experiment details: • Testing: 5%, 10%, 15% salt concentrations • Measuring: plant growth

Step 1: Identify what's being manipulated The scientist is CHANGING salt concentration (5%, 10%, 15%)

Step 2: Identify what's being measured The scientist is MEASURING plant growth

Step 3: Determine independent variable

A) "Plant growth" • This is being measured (dependent variable) ✗

B) "Salt concentration" • This is being changed/manipulated ✓ • The scientist controls this ✓ CORRECT!

C) "Type of plant" • This should be constant (controlled variable) ✗

D) "Amount of water" • Should be constant (controlled variable) ✗

E) "Temperature" • Should be constant (controlled variable) ✗

Answer: B) Salt concentration

Variable types:

  1. Independent (manipulated): • What experimenter changes • Plotted on x-axis • "What I change"

  2. Dependent (responding): • What experimenter measures • Plotted on y-axis
    • "What I observe" • Depends on independent variable

  3. Controlled (constant): • Kept the same across all groups • Examples: temperature, light, plant type • Ensures fair test

3Problem 3hard

Question:

Two students measure the same object's length. Student A records 15.2 cm. Student B records 15.8 cm. The actual length is 15.5 cm. Which statement is most accurate?

F) Both measurements are equally precise G) Student A is more accurate H) Student B's measurement is more precise J) Both students have good accuracy K) The measurements are invalid

💡 Show Solution

Accuracy vs. Precision - crucial distinction in science!

Definitions: • Accuracy: How close to true value • Precision: How close repeated measurements are to each other

Given: • True length: 15.5 cm • Student A: 15.2 cm (error = 0.3 cm) • Student B: 15.8 cm (error = 0.3 cm)

Step 1: Evaluate accuracy Student A: |15.2 - 15.5| = 0.3 cm from true value Student B: |15.8 - 15.5| = 0.3 cm from true value

Both are equally accurate! Both are 0.3 cm off.

Step 2: Evaluate precision Precision requires multiple measurements (not given here) Can't determine precision from single measurements

Step 3: Evaluate options

F) "Both measurements are equally precise" • Can't determine precision from one measurement each ✗

G) "Student A is more accurate" • Both have same error (0.3 cm) ✗

H) "Student B's measurement is more precise" • Can't determine precision from single measurement ✗

J) "Both students have good accuracy" • Both within 0.3 cm of true value ✓ • Reasonably close ✓ CORRECT!

K) "The measurements are invalid" • Small errors are normal ✗ • Measurements are reasonable ✗

Answer: J) Both students have good accuracy

Accuracy vs. Precision analogy: Bullseye target: • Accurate: arrows hit center (close to true value) • Precise: arrows clustered together (consistent) • Ideal: accurate AND precise (clustered at center)

Example scenarios: • High accuracy, low precision: 15.0, 16.0, 15.5 (average close, spread out) • Low accuracy, high precision: 18.0, 18.1, 18.0 (clustered but far from true) • High both: 15.4, 15.5, 15.6 (clustered near true value)